Cloning of Inhibin-α Gene and its mRNA Expression Pattern in the Ovary of Congjiang Xiang Pig

To reveal the relationship between inhibin-α(INHA) gene and the reproductive traits of Congjiang Xiang pig, INHA gene was cloned and sequenced taking the genomic DNA of Congjiang Xiang pigs as templates by polymerase chain reaction(PCR) method.The polymorphisms of INHA gene were tested in Congjiang Xiang pig populations with high-litter size and low-litter size using allele-specific PCR(AS-PCR) method.The expression profile of INHA gene in ovaries was detected from Congjiang Xiang pigs with high-litter yiled or low-litter yiled by Real-time PCR method.The complete coding region of INHA gene was 1095 bp in length, which coded for 364 amino acid residues.Compared with the known sequence, two candidate sites, G359A and A373G, were found out from exon 2 region of INHA gene in Congjiang Xiang pig.After investigation for the two sites in a large population, the frequency of alleles between two populations was not significant and without obvious relativity with the litter yiled of Congjiang Xiang pig.However, the INHA mRNA level in the ovary of Congjiang Xiang pig with high-litter yiled was higher than that with low-litter yiled.It suggested that INHA gene was much conserved, INHA gene expression level might be concerned for the regulation of ovary growth and follicle development in Congjiang Xiang pig breed.     

影响‘小凤兰’根状茎增殖、分化和壮苗的因素研究

以‘小凤兰’根状茎为试材,研究了影响‘小凤兰’根状茎增殖、分化、生根壮苗的因素。结果表明:MS基本培养基、蔗糖30g/L、6-BA 2.0mg/L+NAA 0.5mg/L、活性炭0.5g/L有利于根状茎增殖,而添加20%椰汁和番茄汁40g/L会抑制根状茎增殖。1/2MS基本培养基、蔗糖30g/L、6-BA 2.0mg/L+NAA 0.2mg/L、20%椰汁、1 000lx光照强度有利于根状茎分化,不加活性炭有利于芽分化,但抑制根分化,而添加活性炭则抑制芽分化,促进苗分化。添加土豆泥60g/L显著促进试管苗生长,3~4cm高的苗在培养基1/2MS+6-BA 0.1mg/L+NAA0.5mg/L+蔗糖20g/L+土豆泥60g/L+卡拉粉8g/L+活性炭0.5g/L中培养40d即可移栽。     

不同基因型大麦苗期对干旱胁迫的响应及HvPIPs基因表达特征分析

为研究不同基因型大麦苗期对干旱胁迫的响应,挖掘优质抗旱的大麦种质资源,以4份不同基因型大麦(2个高抗品种ZDM5430和ZDM5458,2个干旱敏感型品种7DCADA和IL-12)为材料,利用聚乙二醇(PEG-6000)模拟干旱条件,综合分析不同品种大麦的苗期抗旱性差异;并通过对不同品种根系进行PIPs实时定量分析,研究水通道蛋白AQPs基因与干旱胁迫之间的应答关系。结果表明,持续干旱胁迫会使大麦生长变缓,各处理苗长、根长、叶含水量和根含水量都呈下降趋势,其中,4个品种的大麦叶含水量在干旱胁迫14 d时下降最为显著,与对照相比,其降幅分别为29.14%、52.64%、21.67%和72.15%。干旱胁迫对大麦苗期干物质积累量和根冠比的影响较小,各处理间差异不显著;但持续干旱后,抗旱品种ZDM5430的根冠比呈上升趋势,在胁迫7、14、21 d时较CK分别增加了4.92%、42.86%和21.05%。随着胁迫时间的延长,大麦旗叶叶绿素含量和根系活力也呈下降趋势,其中ZDM5430和ZDM5458较对照组降幅较小,说明抗旱品种的形态指标、叶绿素含量及根系活力受干旱胁迫的影响较小。通过PIP...     

Design of the PC/104 Bus Mode Multi-channel Analyzer

This paper expatiates on the bus structure and develop the bus mode Multi-Channel Analyzer (MCA). The nucleus of this MCA is the PC/104 embedded computer, and it introduces the bus communication to realize system and data communication. It may realize data collecting and showing in the field, because the bus mode is rapid. At first, on the hardware we introduce CMOS components, this reduces power, volume and weight. These make it adapts to use in the field; besides, on the software we adopt C and assembly language to program in order to improve conversion speed. At the same time, single-task DOS operating system is infroduced, which makes the software run more steadily, operate more simply. By means of testing on the spot it can normally work, so it suitable to work in the field.     

摆动式花生收获机整体载荷的试验与分析

从理论上建立了摆动式花生收获机的空间力系模型.在此基础上,围绕摆动式花生收获机的主要试验参数-传动速比、偏心距、栅条长度与前进速度的改变进行了摆动式花生收获机整体载荷的试验.试验证明:花生收获机在工作时所受载荷平衡,通过二次回归旋转组合试验测定整体载荷随4因素的变化规律是显著的,结合花生收获机的实际工作条件分析了回归方程的适用范围.本试验为摆动式花生收获机在功率消耗、力的平衡等方面的进一步改进提供了依据.     

华北冬小麦-夏玉米农田水分动态模拟研究

冬小麦-夏玉米连作是华北地区主要的粮食作物种植模式。根据华北季节性冻土区的特点,将全年划分为作物生长期与越冬期,分别建立了作物生长条件下农田水分运移模型、冻融条件下土壤水热运移模型。前一模型主要包括参照腾发量计算、腾发量分配、作物根系吸水、土壤表面蒸发、土壤水分特征参数和土壤水分运动等子模型;后一模型主要包括冻土水热耦舍迁移、地气水热交换等子模型。应用以上模型对冬小麦-夏玉米连作条件下的土壤水分过程进行模拟,根据北京永乐店试验资料对模型进行检验。模拟了不同降水水平年、不同灌溉处理下的农田灌溉制度及土壤水分过程,分析了降水、灌溉对农田蒸散、土壤水利用、深层渗漏等的影响。     

陕西关中灌区管理体制改革成效分析

2000～2004年连续5年对陕西9大灌区120条定点斗渠与800个定点农户进行入户调查,对管理体制改制进程及与农户灌溉情况、水费支出、农业投资与收入等进行了入户监测评价调查,结果表明改制斗渠系状况良好,灌溉水利用系数、农户收入增加,农户实际水费支出减少.管理体制改革带给农户的好处是显而易见的.     

阜新县节水农业分区研究

针对阜新县水资源紧缺的现状，在综合分析全县气候、土壤、水资源现状、农业种植制度和经济发展水平等因素的基础上，对阜新县节水型农业分区进行了研究，为确保分区指标的科学性、系统性，采用模糊数学聚类分析方法，探讨节水农业的地域分布规律，进行节水农业分区，以阜新县为例对因地制宜地发展东北半干旱地区的节水农业分区进行有益的探索。     

3S及其集成技术在灌区信息管理中的应用

介绍了3S技术在灌区信息管理中的研究现状,指出了灌区管理信息化建设的重要性,提出了以RS、GPS技术信息采集为基础,以高速安全可靠的计算机网络为手段,构建RS、GIS和GPS技术功能为一体的灌区信息管理系统的方法,并初步探讨了3S技术在灌区信息管理中的应用前景和发展趋势。